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Exosomes Therapy

Exosomes are microscopic, structured particles produced during cell culture. Exosomes travel in body fluids such as blood, urine, saliva, etc. Each Exosome molecule is 40 to 150 nanometers (nm) in size, with an internal structure containing genetic signals such as DNA, RNA, miRNA (microRNA), proteins, growth factors and is surrounded by a lipid bilayer and a surface protein layer. Exosomes receive genetic material and growth factors from the original cell, after being secreted, they will travel through body fluids to target cells and secrete signals to exchange information with the target cell. Exosomes play an important role in promoting communication between substances to target cells in the body, thereby promoting the healing process, repairing damaged tissues and cells (cell proliferation, angiogenesis and immune regulation).

Exosomes are being increasingly applied in medicine and especially in cosmetic dermatology because of their high treatment effectiveness without being invasive.

Some outstanding features

Cytokine

These are very small proteins that function to transmit information to cells and exert various effects. So far, hundreds of cytokines have been discovered, and mesenchymal stem cells also contain from dozens to about 200 cytokines depending on the cell type.

On the side of the cell that receives cytokines, there are specific structures that capture each cytokine, called receptors. Cytokines and receptors have a close compatibility that can be compared to a “key-lock relationship”, which is a common feature in humans that does not differ between individuals, but there is no compatibility between different animal species. That is why “human-derived cytokines” can be expected to have the best therapeutic effect on the human body.

Excerpt from *Epidermal growth factor; PDB-101

Exosomes

This is a membrane-enclosed, sac-like structure released from cells, also known as an Extracellular Vesicle. It contains a large number of molecules such as proteins, lipids, and RNA, etc., and when exomes are taken up by cells, these molecules are also taken up at the same time. It has been shown that the components contained in exosomes differ depending on the type and state of the cell releasing them.

The report suggests that exosomes play an important role as a vehicle for transmitting information to cells through routes other than cytokines. Discoveries in recent years have overturned conventional medical understanding, the medical application of exosomes is still being widely studied, and there are high expectations that it will play a major role in the next generation of regenerative medicine.

Relationship between stem cells and culture supernatant

Previously, it was believed that stem cells could effectively treat many diseases by replacing cells damaged by disease or aging. However, recent studies have shown that in stem cell therapy, the functional components secreted by mesenchymal stem cells will act on surrounding cells to repair them. The effect of functional components (cytokines, exosomes, etc.) on surrounding cells is called the paracrine effect. It can be said that culture supernatant therapy is a treatment method that only extracts and utilizes the paracrine effect of stem cell therapy without involving the stem cells themselves.

Therapy using culture supernatants does not contain stem cells and is therefore not classified as regenerative medicine under current law in Japan (Regenerative Medicine Safety Assurance Law). However, it is expected to play a leading role in next-generation regenerative medicine as a cell-free therapy, meaning it does not require stem cells.

The composition of culture supernatant (the components that make up the culture supernatant) varies greatly depending on the type of stem cell and culture conditions, etc. Therefore, it is important to produce it using cell culture under strict safety and environmental management to achieve therapeutic effects. Our clinic has established a research system in collaboration with cell processing facilities, and is conducting treatment using culture supernatant in a synchronized manner from production to clinical use.

Reported cases of treatment with adipose-derived stem cells

The efficacy of adipose-derived stem cells for various diseases has been reported in basic and clinical studies both in and outside Japan. *Our clinic has been granted a registration number to provide intravenous infusion therapy for the treatment of premenopausal disorders and chronic pain, and local injection therapy for the treatment of age-related facial changes.

Cerebral infarction
Concussion
Spinal cord injury
Multiple sclerosis
Hypoxic ischemic encephalopathy
Parkinson's disease
Alzheimer's disease
ALS (amyotrophic lateral sclerosis)
Polio (infantile paralysis)
Peripheral neuropathy
Chronic pain
Knee arthritis
Tendon injury
Arthritis
Difficult to heal fracture
Palmar and plantar pustules
Atopic dermatitis
Acne scars
Vitiligo
AGA (male pattern hair loss)
Other hair loss
Myocardial infarction, angina
Arteriosclerosis

Peripheral Arterial Disease
Diabetes
Chronic kidney disease
Liver disease (hepatitis, cirrhosis, liver damage, etc.)
Primary biliary cirrhosis
Emphysema
Pulmonary fibrosis
Chronic obstructive pulmonary disease
Autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosus, etc.)
GVHD (graft-versus-host disease)
Female premenopausal disorders
Male menopause disorders
Prostate enlargement
Erectile dysfunction
Urinary incontinence
Bone defects
Osteoporosis
Periodontal disease
Post-breast cancer surgery (breast reconstruction)
Breast augmentation
Anti-aging treatment (skin rejuvenation, blood vessels, etc.)
New coronavirus infection
Sequelae after contracting the new coronavirus infection

Health

Administered throughout the body through a vein (intravenous infusion)

It can be expected to have the effect of improving various diseases, preventing aging, improving cold sensitivity, restoring eyesight, restoring sleep disorders, restoring male function and improving blood circulation, etc.

Delivered to the injured area or joints (local injection)

By injecting directly into the damaged or painful area, cells are activated, tissue is repaired and improved, and inflammation suppression and pain relief can be expected.

Beautify

Subcutaneous injection (local injection)

It can be expected to have a rejuvenating effect on the skin against age-related changes such as age spots, wrinkles and sagging. In addition, by injecting into the scalp it can be expected to have a stimulating effect on hair growth, improving hair quality and graying hair.

Stem cell therapy

Stem cells (SC) are pluripotent cells. In a certain environment, they have the ability to self-regenerate and differentiate according to certain functions.

Blood Purification

Blood purification therapy is an advanced treatment that helps prevent atherosclerosis, which can lead to strokes and heart attacks. It removes harmful substances from the blood and provides anti-aging benefits to blood vessels.

Health Forecast

We provide a breakthrough solution to address aging concerns through advanced blood protein analysis. Our digital health service in Japan not only enables accurate detection and prediction of age-related conditions such as dementia, CKD, and various forms of stroke, but also prioritizes patient education for appropriate lifestyle changes. This technology aims to detect the possibility of future mortality so that customers can take early intervention and proactively prevent and protect their health to minimize the burden of disease and healthcare costs in the future.

NMN (NAD+) Therapy

NMN (full name is Nicotinamide Mononucleotide, also known as NAD+ precursor) is a key intermediate in the synthesis of Nicotinamide Adenine Dinucleotide (NAD+) – an essential enzyme for maintaining various important functions of the cell. Including metabolism, DNA repair, growth stimulation and maintaining cell survival.

Frequently Asked Questions

What are stem cells? What are the properties of stem cells?

WHAT ARE STEM CELLS?

Stem cells (SC) are multipotent cells. In a certain environment, they have the ability to self-regenerate and differentiate according to certain functions. According to the body's natural mechanism, when any cell or tissue is damaged, stem cells will automatically concentrate and move to that location to heal and restore the damage.

Stem Cell Technology brings outstanding value to the “Regenerative Medicine Technology” industry, with the ability to regenerate, replace, and repair defective and damaged cells. Stem cells are isolated and cultured in vitro to create healthy cells and then reinjected into the body.
human body is sampled. By this method, new cells will replace and repair damaged or dead cells. Therefore, Stem Cell Medical Technology is being used to treat incurable and dangerous diseases.

STEM CELL CHARACTERISTICS

Feature 1: Differentiation feature (Cells maintain function by undergoing symmetric and asymmetric division.)
Feature 2: Multi-functionality( Cells have the ability to differentiate into many types of cells. Including: unipotent cells, multipotent cells, and multipotent cells.)
Feature 3: Low autoimmunity( Stem cells are primitive cells that have not yet differentiated.)
Feature 4: Compatibility( Compatible with different bodies.)

What are the uses of stem cells?

Treatment of eye diseases:

In clinical studies and trials such as: macular degeneration, retinitis and optic nerve disorders.

Stem cells have the ability to repair and replace eye tissue cells and restore vision and function to the eyes.

Treatment of lung and respiratory diseases:

Perform autologous stem cell treatment for asthma, bronchitis, emphysema causing heart rhythm disorders.

Treatment of kidney disease:

Basic studies have shown that stem cells have the ability to differentiate differentiate into kidney-specific cells and renal parenchymal cells.
Therefore, the transplanted stem cells will successfully repair, replace or regenerate the kidney to maintain its working function.

Treatment of diseases related to the immune system:

Improve symptoms of immune-related diseases by using Stem Cells to regenerate the immune system, improve immune regulation of: liver disease, lupus erythematosus, rheumatoid arthritis and type 1 diabetes.

Cardiovascular disease:

Mainly atherosclerosis, vasculitis, vascular dysfunction, angiogenesis, myocardial infarction, etc. The most common is atherosclerosis.

Gynecological disorders:

Stem cells are one of the effective methods of treating infertility. Applied to some gynecological diseases: ovarian failure, endometrial damage.

Other:

Stem cells have the ability to repair, replace and improve the function of diseases of teeth, hair, skin wounds, endocrine disorders through the skin, and treat diseases of ectodermal function.

Stem cell sampling and culture process?

Adipose tissue stem cells are extracted fat that is separated, purified, cultured and cloned, used as precursor cells for fibroblasts, injected into the subcutaneous epidermis, below the blood vessels. By repairing, replacing, bringing about effective, improved skin, erasing wrinkles and scars.

TRIP 1

The client underwent cell sampling (mesenchymal fat cells) in the area behind the ear which took about 30 minutes.
Performing cell sampling behind the ear will ensure better aesthetics than taking it from the umbilical cord area.

TRIP 2

The client injects the selected stem cells into the body by drip or injection.
cultured from previously collected cell samples.
The procedure takes a maximum of 2 hours and the client can resume normal activities immediately.

Why should you choose Riso Clinic to provide stem cell services?

No. Stem cell therapy is an advanced technology, and facilities providing stem cell therapy must meet strict standards set by the Japanese government and must be licensed to operate. Riso Clinic is a regenerative medicine provider officially certified by the Ministry of Health, Labor and Welfare of Japan.

How many stem cells will be injected per treatment? Is there a limit?

In one treatment, you can get between 100 million and 200 million cells at a time. It can be injected intravenously, injected into the face and knees at the same time. In such cases, up to 200 million cells can be injected into each area.

How are stem cell samples collected?

Stem cells are harvested through a small incision of just a few millimeters in the abdomen or behind the ear. We use anesthesia, so there is no pain and no need for hospitalization. Patients can go home the same day.

Does stem cell therapy have any side effects?

We use the customer's own stem cells, so side effects are negligible. We strictly follow the safety regulations set by the Japanese government.

Is there any age limit for treatment?

There is no specific age limit, but it is recommended to receive treatment as early as possible. Receiving multiple treatments early can enhance the anti-aging effects and provide better results in repairing damaged tissues and preventing disease.

How many stem cells are collected per sample?

In one harvest, we can produce four aliquots of stem cells. Each aliquot can grow up to 400 million stem cells. Once aliquots are used, they cannot be reused. Once all of these aliquots are used up, a new sampling must be performed.

How many times do I need to go to Japan?

If this is your first time using stem cell therapy, you will need to come to Japan twice — once to have your stem cells collected and once to have the stem cells injected into your body. After the initial sample is collected, you will not need to have further samples taken until all of the stem cells collected have been used.

How long does it take between sample collection and stem cell injection?

At least one month. After the cell sample is collected, it takes about one month for the cells to grow and develop to the required number. You need to schedule your injection and confirm it at least one month in advance of your desired treatment date.

How long does it take to take a cell sample?

Cell sampling time takes about 30 minutes.

Do I need to rest after having a cytology sample taken?

Depending on your health condition, you can move around normally.

How long does stem cell infusion take?

Stem cell infusion time is about 2 hours.

Do I need to rest after a stem cell infusion?

Depending on your health condition, you can move around normally. Avoid strenuous activities such as sports for 3 days from the date of implementation.